An Improved Surgical Approach for Complete Interhemispheric Corpus Callosotomy Combined with Extended Frontoparietal Craniotomy in Mice

Callosotomy is an invasive method that used to study the role of interhemispheric functional connectivity in brain. This surgical approach technically demanding perform small laboratory animals, such as rodents, due several methodological challenges. To date, there exist two main approaches for transecting corpus callosum (CC) rodents: trephine hole(s) or unilateral craniotomy, which cause damage cerebral cortex injury large vessels, and may lead intracranial hemorrhage animal death. presents improved complete callosotomy mice using combined with bilateral extended craniotomy across midline. demonstrated provided visual space required hemisphere sinus retraction, thus keeping blood vessels surrounding brain structures intact under microscope standardized instruments. We also emphasized importance good post-operative care leading increase overall survival following experimentation. optimized avoids extracallosal tissue medium- large-sized vessel mice, can provide higher reproducibility/validity among animals when revealing CC various neurological pathologies.